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肿瘤坏死因子相关凋亡诱导配体(TRAIL)重组蛋白

2016-01-18

肿瘤坏死因子相关凋亡诱导配体(TRAIL)重组蛋白

Recombinant Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism speciesCanis familiaris; Canine (Dog)
Product No.klA139Ca01
SourceProkaryotic expression
HostE.coli
Purity> 95%
UOM50ug
Predicted Molecular Mass32.6kDa
Concentrationn/a
ApplicationsSDS-PAGE; WB; ELISA; IP.
Endotoxin Level<1.0EU per 1μg (determined by the LAL method)
ResiduesThr36~Gly281 with two N-terminal Tags, His-tag and T7-tag

FormulationSupplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

肿瘤坏死因子相关凋亡诱导配体(TRAIL)重组蛋白 

SEQUENCES

The sequence of the target protein is listed below.
TRELK QMQDKYSQSG IACFLKEDDI PWDPSDEESM NNPCWQVKWQ LRQFVRKMIL KTYEETIPTA PEKQLNIPYV VSDRGSQRVA AHITGTSRRS MFPIPSSKND KALGHKINSW DSTRKGHSFL NNLHLRNGEL VIHQRGFYYI YSQTYFRFQE PEEIPTGQNR KRNKQMVQYI YKHTSYPDPI LLMKSARNSC WSKDSEYGLY SIYQGGIFEL KENDRIFVSV SNEQLIDMDQ EASFFGAFLI G

USAGE

Reconstitute in sterile PBS, pH7.2-pH7.4.

肿瘤坏死因子相关凋亡诱导配体(TRAIL)重组蛋白

STORAGE AND STABILITY

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

肿瘤坏死因子相关凋亡诱导配体(TRAIL)重组蛋白About the MARKER (complimentary)

Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.